Fig 1: A scheme for proposed mechanisms for canagliflozin-induced suppression of cell proliferation in Hep3B cell.Abbreviations: SGLT2, sodium-glucose cotransporter 2; ADP, adenosine diphosphate; ATP, adenosine triphosphate; AMPK, AMP-activated protein kinase; SCD, stearoyl-CoA desaturase; ACC, acetyl-CoA carboxylase; NME1, nucleoside diphosphate kinase1; NDP, nucleotide diphosphate; NTP, nucleotide triphosphate; PRIM2, DNA primase, polypeptide 2; DNA, deoxyribonucleic acid.
Fig 2: Expression of SGLT2 in Hep3B and Huh7.A) Immunoblotting using 4 different antibodies for SGLT2: Antibody-a, Proteintech Group, Inc. (24654-1-AP); Antibody-b, Abcam plc. (ab137207); Antibody-c, Abcam plc. (ab85626), Antibody-d, Cell Signaling Technology, Inc. (#14210). B) Immunohistochemistry for SGLT2. Green indicates SGLT2. The nucleus was stained by DAPI. Scale bar = 50 μm. C) Double immunostaining for mitochondrial PDK-1 and SGLT2. Green indicates mitochondrial PDK-1. Red indicates SGLT2. Yellow indicates colocalization of mitochondrial PDK-1 and SGLT2. The nucleus was stained by DAPI. Scale bar = 50 μm. D) Immunoblotting for SGLT2 in mitochondrial and cytoplasmic fractions. Abbreviations: SGLT2, sodium-glucose cotransporter 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; DAPI, 4',6-diamidino-2-phenylindole; PDK-1, pyruvate dehydrogenase kinase 1.
Fig 3: RH reduced brain GLUT3-mediated glucose uptake in STZ-induced APP/PS1-DM mice.(A) Representative PET/CT images showing in vivo brain 18F-FDG uptake (coronal, sagittal, and transaxial sections, n = 6 mice for each group). Cor, Cortex; Hip, hippocampus; Mid, midbrain; HPOA, hypothalamus; BS, brain stem; Cer, cerebellum; OB, Olfactory Bulb; BF, basal forebrain; Tha, thalamus; SC, superior colliculi; CG, central gray; Str, striatum. (B) Quantification for SUV of 18F-FDG in brain region (n = 6 for mice for each group). (C) Western blot and quantitation for GLUT1, GLUT2, GLUT3, GLUT4, GLUT5, SGLT1, and SGLT2 in hippocampal homogenates (n = 3 mice for each group). The data are expressed as the mean ± SEM. Statistical significance was assessed using unpaired Student’s t test. *P < 0.05 and **P < 0.01, APP/PS1-DM versus APP/PS1; #P < 0.05 and ##P < 0.01, APP/PS1-DM-RH versus APP/PS1-DM; WT versus APP/PS1; NS, no significant difference.
Supplier Page from Abcam for Anti-SGLT2 antibody